The report was generated using the JSEQ_scRNAseq© single-cell analysis pipeline

For more information, please visit: https://github.com/jkubis96/JSEQ_scRNAseq/tree/v2.3.2


Cell content analysis



The number of genes and counts per cell



The ratio of the number of genes to counts



The percentage of ribosomal and mitochondrial genes [%]



Quality control of cell content


Number of counts



Mitochondrial gene content



Ribosomal gene content



Number of cells at different stages of the analysis



Top highly variable genes in the dataset


##  [1] "LINC00486"       "ENSG00000225718" "DNAJC27-AS1"     "COL1A2"         
##  [5] "COL3A1"          "C10orf67"        "DLX6-AS1"        "BACH2"          
##  [9] "HS6ST3"          "LINC00536"       "IGFBP5"          "ENSG00000286662"
## [13] "FAM169A"         "POSTN"           "ENSG00000289578" "MEG3"           
## [17] "TOP2A"           "NRP2"            "ENSG00000223393" "ENSG00000253300"




Selection of analysis parameters


The cutoff for the number of principal components


ElbowPlot


JackStrawPlot for 26 PCs


The results of cell grouping

UMAP processing and visualization of cell clusters

Markers selected for the clusters
cluster p_val avg_logFC pct.1 pct.2 p_val_adj gene subclass
0 0.00e+00 1.632725 0.278 0.134 0.0000000 CNBD1 MGE SOX6 CNBD1
0 0.00e+00 1.423641 0.247 0.111 0.0000000 SMYD3 MGE SOX6 CNBD1
0 0.00e+00 1.822053 0.162 0.044 0.0000088 ENSG00000287277 MGE SOX6 CNBD1
0 1.00e-07 1.574893 0.149 0.059 0.0013372 ARNT2 MGE SOX6 CNBD1
0 1.00e-07 1.746670 0.118 0.049 0.0022883 RFTN1 MGE SOX6 CNBD1
0 2.00e-07 1.485455 0.152 0.054 0.0036370 GATB MGE SOX6 CNBD1
0 1.10e-06 1.861306 0.108 0.031 0.0226808 CAD MGE SOX6 CNBD1
0 2.60e-06 1.358487 0.108 0.059 0.0543285 GREB1 MGE SOX6 CNBD1
0 5.50e-06 1.437100 0.105 0.046 0.1144458 TLN2 MGE SOX6 CNBD1
0 1.51e-05 1.435094 0.103 0.039 0.3143854 ASIC2 MGE SOX6 CNBD1
1 0.00e+00 1.819840 0.231 0.017 0.0000000 FNBP4 MGE SOX6 FNBP4
1 0.00e+00 2.250200 0.211 0.015 0.0000000 FTLP2 MGE SOX6 FNBP4
1 0.00e+00 1.944465 0.182 0.011 0.0000000 CD46 MGE SOX6 FNBP4
1 0.00e+00 2.082309 0.178 0.013 0.0000000 FTLP3 MGE SOX6 FNBP4
1 0.00e+00 4.920143 0.102 0.000 0.0000000 PNRC1 MGE SOX6 FNBP4
1 0.00e+00 1.860129 0.132 0.008 0.0000000 VCAN MGE SOX6 FNBP4
1 0.00e+00 2.257860 0.109 0.004 0.0000002 HES1 MGE SOX6 FNBP4
1 0.00e+00 1.824431 0.102 0.004 0.0000013 COPB2 MGE SOX6 FNBP4
1 0.00e+00 1.879569 0.106 0.006 0.0000043 ZNF24 MGE SOX6 FNBP4
1 0.00e+00 1.665797 0.102 0.008 0.0000663 UBR5 MGE SOX6 FNBP4
2 0.00e+00 4.052787 0.341 0.013 0.0000000 CARMIL1 MGE LHX8 BACH2
2 0.00e+00 3.243193 0.400 0.032 0.0000000 HS6ST3 MGE LHX8 BACH2
2 0.00e+00 4.990224 0.235 0.006 0.0000000 FDX1 MGE LHX8 BACH2
2 0.00e+00 3.030392 0.376 0.056 0.0000000 BACH2 MGE LHX8 BACH2
2 0.00e+00 6.613454 0.129 0.000 0.0000001 LINC01937 MGE LHX8 BACH2
2 0.00e+00 3.487471 0.141 0.004 0.0000370 DNAH2 MGE LHX8 BACH2
2 0.00e+00 3.932459 0.129 0.003 0.0000665 SLC9A7P1 MGE LHX8 BACH2
2 0.00e+00 4.667064 0.118 0.003 0.0003223 ADAM18 MGE LHX8 BACH2
2 1.00e-07 4.193026 0.118 0.004 0.0012765 PHYH MGE LHX8 BACH2
2 2.00e-07 3.132183 0.141 0.012 0.0032755 TYW1B MGE LHX8 BACH2



Cluster classification and naming based on markers


Subclasses of cells


The principal component plot



The UMAP plot



Presentation of subtypes identified with the CSSG algorithm


Quality control for cell subtypes


The principal component plot



The UMAP plot



The HDMAP plot



Markers selected for the subtypes

p_val avg_logFC cluster gene
0.0000000 2.9775419 MGE SOX6 CNBD1 - CSMD1 CSMD1
0.0067680 2.5995563 MGE SOX6 CNBD1 - CSMD1 PGLYRP2
0.0000007 4.1187383 MGE SOX6 FNBP4 - RPL21 RRP8
0.0000207 4.2176488 MGE SOX6 FNBP4 - RPL21 ENSG00000226851
0.0001131 4.3096828 MGE SOX6 FNBP4 - RPL21 AUP1
0.0001131 4.1431148 MGE SOX6 FNBP4 - RPL21 ELOBP2
0.0006231 4.3269169 MGE SOX6 FNBP4 - RPL21 NDUFV2P1
0.0006231 4.1137395 MGE SOX6 FNBP4 - RPL21 NEMP1
0.0034893 4.0277027 MGE SOX6 FNBP4 - RPL21 CALB2
0.0034893 4.1900422 MGE SOX6 FNBP4 - RPL21 TRPM4
0.0034893 4.2457325 MGE SOX6 FNBP4 - RPL21 RBP1
0.0034893 4.2843434 MGE SOX6 FNBP4 - RPL21 NTF3
0.0000462 4.3349635 STR FOXP1 FNBP4 - MAP1B CROCCP3
0.0004974 4.1827562 STR FOXP1 FNBP4 - MAP1B ENSG00000261094
0.0004974 4.6634531 STR FOXP1 FNBP4 - MAP1B IL17RE
0.0055157 4.1852937 STR FOXP1 FNBP4 - MAP1B RORB-AS1
0.0055157 4.3391063 STR FOXP1 FNBP4 - MAP1B CARMIL3
0.0055157 4.1755203 STR FOXP1 FNBP4 - MAP1B TEX30
0.0055157 4.2708686 STR FOXP1 FNBP4 - MAP1B ZNF606
0.0055157 4.3969406 STR FOXP1 FNBP4 - MAP1B PPP1R13B
0.0055157 4.2440965 STR FOXP1 FNBP4 - MAP1B APAF1
0.0055157 4.8636031 STR FOXP1 FNBP4 - MAP1B ENSG00000287065
0.0000140 6.3346873 MGE LHX8 BACH2 - ZFPM2 ENSG00000236453
0.0000140 5.0607620 MGE LHX8 BACH2 - ZFPM2 MFAP3
0.0002049 5.2687120 MGE LHX8 BACH2 - ZFPM2 OCM
0.0002049 5.2382015 MGE LHX8 BACH2 - ZFPM2 ENSG00000283480
0.0002049 5.0970528 MGE LHX8 BACH2 - ZFPM2 WNT7B
0.0002049 5.0045331 MGE LHX8 BACH2 - ZFPM2 ENSG00000288684
0.0030729 5.0385243 MGE LHX8 BACH2 - ZFPM2 ENSG00000287347
0.0030729 4.9941999 MGE LHX8 BACH2 - ZFPM2 GTF2IP13
0.0030729 5.4745555 MGE LHX8 BACH2 - ZFPM2 MTA1
0.0030729 7.3380599 MGE LHX8 BACH2 - ZFPM2 DDI1
0.0000992 7.2818571 MGE SOX6 FNBP4 - RBBP6 SEL1L3
0.0000992 6.2916171 MGE SOX6 FNBP4 - RBBP6 CACNG5
0.0003504 3.8286049 MGE SOX6 FNBP4 - RBBP6 ITGA6
0.0034854 4.3555206 MGE SOX6 FNBP4 - RBBP6 NOMO1
0.0034854 3.8372130 MGE SOX6 FNBP4 - RBBP6 PMS2P1
0.0034854 3.8212474 MGE SOX6 FNBP4 - RBBP6 MTO1
0.0059029 3.9888510 MGE SOX6 FNBP4 - RBBP6 OGFR
0.0059029 4.1423272 MGE SOX6 FNBP4 - RBBP6 XKR7
0.0089032 4.6161275 MGE SOX6 FNBP4 - RBBP6 TRMT9B
0.0089033 3.8483364 MGE SOX6 FNBP4 - RBBP6 FAM210B
0.0000000 2.4901440 Unknow CNBD1 - APIP APIP
0.0012546 0.4723499 Unknow CNBD1 - APIP LINC01681
0.0016739 1.9740184 Unknow CNBD1 - APIP ENSG00000260364
0.0085553 1.9951770 Unknow CNBD1 - APIP ENSG00000288692
0.0093792 2.5445637 Unknow CNBD1 - APIP PLA2R1
0.0088856 5.7578178 STR FOXP2 FNBP4 - LINC00632 CT66
0.0088856 5.7578178 STR FOXP2 FNBP4 - LINC00632 ENSG00000231271
0.0088856 5.7578178 STR FOXP2 FNBP4 - LINC00632 ENSG00000253683
0.0088856 5.7578178 STR FOXP2 FNBP4 - LINC00632 RNF32-AS1
0.0088856 5.7578178 STR FOXP2 FNBP4 - LINC00632 SH2D7
0.0088856 5.7578178 STR FOXP2 FNBP4 - LINC00632 SLC47A1
0.0088856 5.7578178 STR FOXP2 FNBP4 - LINC00632 ZNF45-AS1
0.0088856 6.7142255 STR FOXP2 FNBP4 - LINC00632 DMXL1-DT
0.0088856 6.7142255 STR FOXP2 FNBP4 - LINC00632 ENSG00000254975
0.0088856 6.7142255 STR FOXP2 FNBP4 - LINC00632 ENSG00000286536
0.0088856 6.7142255 STR FOXP2 FNBP4 - LINC00632 LINC02387
0.0088856 6.7142255 STR FOXP2 FNBP4 - LINC00632 PLUT
0.0088856 6.4869574 STR FOXP2 FNBP4 - LINC00632 ENSG00000287783
0.0088856 5.7953322 STR FOXP2 FNBP4 - LINC00632 CATSPER2P1
0.0088856 5.7953322 STR FOXP2 FNBP4 - LINC00632 ENSG00000289410
0.0088856 5.7953322 STR FOXP2 FNBP4 - LINC00632 MFSD14A
0.0017174 5.5642059 MGE LHX8 CNBD1 - SMYD3 ATG9B
0.0017174 5.8161674 MGE LHX8 CNBD1 - SMYD3 ANGPT4
0.0017174 5.5489578 MGE LHX8 CNBD1 - SMYD3 CASP10
0.0069030 4.5628689 MGE LHX8 CNBD1 - SMYD3 CFAP99
0.0069030 4.7824916 MGE LHX8 CNBD1 - SMYD3 PITRM1-AS1
0.0069030 4.5821495 MGE LHX8 CNBD1 - SMYD3 ENSG00000257729
0.0069030 4.7854424 MGE LHX8 CNBD1 - SMYD3 ANXA3
0.0069030 4.1868678 MGE LHX8 CNBD1 - SMYD3 UBAC2-AS1
0.0069030 4.1447242 MGE LHX8 CNBD1 - SMYD3 SIM2
0.0069030 4.4957132 MGE LHX8 CNBD1 - SMYD3 PITX3
0.0080382 6.4050049 MGE SOX6 FNBP4 - CWC25 ENSG00000280193
0.0080382 6.4050049 MGE SOX6 FNBP4 - CWC25 LILRB3
0.0080382 6.4050049 MGE SOX6 FNBP4 - CWC25 ZWINT
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 ENSG00000253667
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 ENSG00000267733
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 ENSG00000275318
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 ENSG00000280117
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 ENSG00000283236
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 ENSG00000286236
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 HGH1
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 RAB11FIP5
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 SF3A2
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 SNPH
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 TDRD6
0.0080382 6.1178737 MGE SOX6 FNBP4 - CWC25 YDJC
0.0088856 6.1356557 MGE SOX6 FNBP4 - NOP58 ENSG00000288061
0.0088856 6.1356557 MGE SOX6 FNBP4 - NOP58 IFITM5
0.0088856 6.1356557 MGE SOX6 FNBP4 - NOP58 MIR222HG
0.0088856 6.1356557 MGE SOX6 FNBP4 - NOP58 UNC13D
0.0088856 6.3317994 MGE SOX6 FNBP4 - NOP58 ENSG00000287016
0.0088856 6.0793148 MGE SOX6 FNBP4 - NOP58 ENSG00000176183
0.0088856 6.0793148 MGE SOX6 FNBP4 - NOP58 ENSG00000232035
0.0088856 6.0793148 MGE SOX6 FNBP4 - NOP58 ENSG00000287285
0.0088856 6.0793148 MGE SOX6 FNBP4 - NOP58 MIX23P1
0.0088856 5.9407469 MGE SOX6 FNBP4 - NOP58 LHFPL3-AS2
0.0088856 5.9407469 MGE SOX6 FNBP4 - NOP58 RASA4DP
0.0088856 5.9407469 MGE SOX6 FNBP4 - NOP58 TMPO-AS1
0.0000000 3.4007163 MGE SOX6 FNBP4 - ZNF292 ZNF292
0.0000992 6.0127966 MGE SOX6 FNBP4 - ZNF292 DCAF10
0.0012930 3.2698567 MGE SOX6 FNBP4 - ZNF292 BRAT1
0.0034846 3.5181641 MGE SOX6 FNBP4 - ZNF292 DDX54
0.0034852 3.9187125 MGE SOX6 FNBP4 - ZNF292 ANP32AP1
0.0034852 4.0680895 MGE SOX6 FNBP4 - ZNF292 MCM7
0.0034854 3.8660647 MGE SOX6 FNBP4 - ZNF292 RSPH4A
0.0059015 4.0161103 MGE SOX6 FNBP4 - ZNF292 LMLN
0.0059029 3.6718755 MGE SOX6 FNBP4 - ZNF292 ENSG00000231252
0.0089032 3.3305991 MGE SOX6 FNBP4 - ZNF292 CIAO3
0.0000512 6.5683685 STR FOXP1 FNBP4 - ABCF1 ENSG00000280106
0.0080382 6.4050049 STR FOXP1 FNBP4 - ABCF1 ENSG00000228664
0.0080382 6.4050049 STR FOXP1 FNBP4 - ABCF1 ENSG00000283538
0.0080382 6.4050049 STR FOXP1 FNBP4 - ABCF1 LINC00587
0.0080382 6.4050049 STR FOXP1 FNBP4 - ABCF1 MTFR2
0.0080382 6.4050049 STR FOXP1 FNBP4 - ABCF1 STAT4
0.0080382 6.4443549 STR FOXP1 FNBP4 - ABCF1 ENSG00000239628
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 ENSG00000254859
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 ENSG00000269419
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 ENSG00000279356
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 ENSG00000283462
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 ENSG00000286449
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 FLG
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 GABRA6
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 IL18R1
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 LINC01065
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 SLC7A9
0.0080382 6.2911859 STR FOXP1 FNBP4 - ABCF1 TEKT1
0.0063155 7.4191807 Unknow CNBD1 - ZFAS1 AGT
0.0063155 7.4191807 Unknow CNBD1 - ZFAS1 ENSG00000287038
0.0063155 7.4191807 Unknow CNBD1 - ZFAS1 GBA3
0.0063155 7.4191807 Unknow CNBD1 - ZFAS1 XPNPEP2
0.0063155 7.2537742 Unknow CNBD1 - ZFAS1 AOX3P
0.0063155 7.2537742 Unknow CNBD1 - ZFAS1 CARMN
0.0063155 7.2537742 Unknow CNBD1 - ZFAS1 DSCAS
0.0063155 7.2537742 Unknow CNBD1 - ZFAS1 ENSG00000248417
0.0063155 7.2537742 Unknow CNBD1 - ZFAS1 LINC02413
0.0063155 7.1961871 Unknow CNBD1 - ZFAS1 ENSG00000250791
0.0063155 7.1961871 Unknow CNBD1 - ZFAS1 ENSG00000261734
0.0063155 7.1961871 Unknow CNBD1 - ZFAS1 OR5BA1P
0.0000000 3.1870587 Unknow CNBD1 - CAD CAD
0.0033773 0.9201014 Unknow CNBD1 - CAD SUZ12
0.0034844 4.0738143 Unknow CNBD1 - CAD PHGDH
0.0068735 2.8133869 Unknow CNBD1 - CAD LINC01814
0.0070557 3.1527345 Unknow CNBD1 - CAD ENSG00000287117
0.0088358 2.9573034 Unknow CNBD1 - CAD ANKS1B
0.0088995 3.3957567 Unknow CNBD1 - CAD SYNE3
0.0029699 5.4700878 MGE LHX8 CNBD1 - CNBD1 OR1A1
0.0029699 5.4059815 MGE LHX8 CNBD1 - CNBD1 MUC3A
0.0029699 5.5358450 MGE LHX8 CNBD1 - CNBD1 LINC02032
0.0029699 5.4708538 MGE LHX8 CNBD1 - CNBD1 ENSG00000285703
0.0029699 6.2231153 MGE LHX8 CNBD1 - CNBD1 ENSG00000189229
0.0029699 5.3365875 MGE LHX8 CNBD1 - CNBD1 ENSG00000229269
0.0029699 5.3691658 MGE LHX8 CNBD1 - CNBD1 LINC01844
0.0029699 5.4325339 MGE LHX8 CNBD1 - CNBD1 ENSG00000287183
0.0029699 5.7552514 MGE LHX8 CNBD1 - CNBD1 DMBX1
0.0029699 5.5426414 MGE LHX8 CNBD1 - CNBD1 ENSG00000287876
0.0071823 7.0106906 HIP LHX2 CNBD1 - NASP UBE2Q2P6
0.0071823 6.8467833 HIP LHX2 CNBD1 - NASP C13orf42
0.0071823 6.8467833 HIP LHX2 CNBD1 - NASP CD28
0.0071823 6.8467833 HIP LHX2 CNBD1 - NASP ENSG00000248134
0.0071823 6.8467833 HIP LHX2 CNBD1 - NASP ENSG00000272297
0.0071823 6.8467833 HIP LHX2 CNBD1 - NASP ENSG00000287120
0.0071823 6.8467833 HIP LHX2 CNBD1 - NASP MYL4
0.0071823 6.8467833 HIP LHX2 CNBD1 - NASP PARP9
0.0071823 6.8467833 HIP LHX2 CNBD1 - NASP SRPK3
0.0071823 6.8467833 HIP LHX2 CNBD1 - NASP TREML2
0.0001284 3.9338364 STR FOXP1 CNBD1 - ENSG00000233755 LINC01591
0.0001749 6.0616313 STR FOXP1 CNBD1 - ENSG00000233755 UBL4B
0.0027695 5.5320060 STR FOXP1 CNBD1 - ENSG00000233755 ENSG00000249240
0.0027695 5.8388742 STR FOXP1 CNBD1 - ENSG00000233755 GYG2P1
0.0027695 5.4147100 STR FOXP1 CNBD1 - ENSG00000233755 ENTPD1
0.0027695 5.4022084 STR FOXP1 CNBD1 - ENSG00000233755 C5
0.0027695 5.5209565 STR FOXP1 CNBD1 - ENSG00000233755 ENSG00000286084
0.0027695 5.6353015 STR FOXP1 CNBD1 - ENSG00000233755 GALNT9
0.0027695 5.5085839 STR FOXP1 CNBD1 - ENSG00000233755 ASB18
0.0027695 5.4079417 STR FOXP1 CNBD1 - ENSG00000233755 CKM
0.0000000 2.7459039 CTX NEUROD1 CNBD1 - RPS11 RPS11
0.0011251 4.9413533 CTX NEUROD1 CNBD1 - RPS11 ENSG00000285283
0.0045362 8.2398254 Unknow CNBD1 - GRID2 GP6-AS1
0.0045362 7.7852412 Unknow CNBD1 - GRID2 ENSG00000214888
0.0045362 7.7852412 Unknow CNBD1 - GRID2 ENSG00000225313
0.0045362 7.7852412 Unknow CNBD1 - GRID2 F11-AS1
0.0045362 7.7852412 Unknow CNBD1 - GRID2 KLKB1
0.0045362 7.7852412 Unknow CNBD1 - GRID2 MIF4GD
0.0045362 7.7852412 Unknow CNBD1 - GRID2 NCF4-AS1
0.0045362 7.7852412 Unknow CNBD1 - GRID2 NOXO1
0.0045362 7.7852412 Unknow CNBD1 - GRID2 RNF212
0.0088522 7.5975052 Unknow CNBD1 - GRID2 SOX9-AS1
0.0080382 7.2708567 MGE ETV1 CNBD1 - DYNC1I2 C17orf97
0.0080382 6.8171835 MGE ETV1 CNBD1 - DYNC1I2 C9orf152
0.0080382 6.8171835 MGE ETV1 CNBD1 - DYNC1I2 ENSG00000228436
0.0080382 6.8171835 MGE ETV1 CNBD1 - DYNC1I2 ENSG00000241678
0.0080382 6.8171835 MGE ETV1 CNBD1 - DYNC1I2 ENSG00000253859
0.0080382 6.8171835 MGE ETV1 CNBD1 - DYNC1I2 HSF2BP
0.0080382 6.8171835 MGE ETV1 CNBD1 - DYNC1I2 LINC02820
0.0080382 6.8171835 MGE ETV1 CNBD1 - DYNC1I2 SLC22A18AS
0.0080382 6.6497678 MGE ETV1 CNBD1 - DYNC1I2 CELF2-AS2
0.0080382 6.6497678 MGE ETV1 CNBD1 - DYNC1I2 GFUS
0.0000864 3.5785167 MGE ETV1 BACH2 - ENSG00000254840 LINC01937
0.0001493 2.7972323 MGE ETV1 BACH2 - ENSG00000254840 CACNA2D3
0.0001626 6.4514202 MGE ETV1 BACH2 - ENSG00000254840 ACAD9
0.0005880 4.5004511 MGE ETV1 BACH2 - ENSG00000254840 ENSG00000287893
0.0005880 3.7928026 MGE ETV1 BACH2 - ENSG00000254840 SH2D4B
0.0035753 3.0425094 MGE ETV1 BACH2 - ENSG00000254840 ENSG00000285769
0.0043527 3.1814203 MGE ETV1 BACH2 - ENSG00000254840 SLC9A7P1
0.0056911 2.7864228 MGE ETV1 BACH2 - ENSG00000254840 STX5
0.0088618 4.2772336 MGE ETV1 BACH2 - ENSG00000254840 C1orf216
0.0093876 2.9656339 MGE ETV1 BACH2 - ENSG00000254840 FGFR1
0.0097258 7.1829518 Unknow CNBD1 - RPL39 ADGRD2
0.0097258 6.6938831 Unknow CNBD1 - RPL39 ENSG00000263551
0.0097258 6.6938831 Unknow CNBD1 - RPL39 ENTPD6
0.0097258 6.6938831 Unknow CNBD1 - RPL39 EPB42
0.0097258 6.6938831 Unknow CNBD1 - RPL39 HRK
0.0097258 6.6938831 Unknow CNBD1 - RPL39 IL36B
0.0097258 6.6700076 Unknow CNBD1 - RPL39 ANKRD65
0.0097258 6.6700076 Unknow CNBD1 - RPL39 EBI3
0.0097258 6.6700076 Unknow CNBD1 - RPL39 ECM1
0.0097258 6.6700076 Unknow CNBD1 - RPL39 ENSG00000258661
0.0097258 6.6700076 Unknow CNBD1 - RPL39 ENSG00000260198
0.0097258 6.6700076 Unknow CNBD1 - RPL39 ENSG00000285535
0.0097258 6.6700076 Unknow CNBD1 - RPL39 ENSG00000286377
0.0097258 6.6700076 Unknow CNBD1 - RPL39 ENSG00000288828
0.0000512 6.9728245 Unknow BACH2 - SLC12A2 CLCN7
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 CIB3
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 ENSG00000235126
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 ENSG00000258399
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 ENSG00000279942
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 GUSBP12
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 KLF17
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 LINC01685
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 NFE2L1-DT
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 PCAT6
0.0080382 6.6621744 Unknow BACH2 - SLC12A2 RAMP1
0.0080382 6.7536297 Unknow BACH2 - SLC12A2 ENSG00000230628
0.0080382 6.6810784 Unknow BACH2 - SLC12A2 CFHR3
0.0080382 6.6810784 Unknow BACH2 - SLC12A2 LINC01819
0.0080382 6.6810784 Unknow BACH2 - SLC12A2 LYPD5
0.0080382 6.6810784 Unknow BACH2 - SLC12A2 MYRFL
0.0080382 6.6810784 Unknow BACH2 - SLC12A2 SELENOV
0.0080382 6.6810784 Unknow BACH2 - SLC12A2 SLC35A4
0.0054350 7.3625204 Unknow CNBD1 - SYF2 ENSG00000241754
0.0054350 7.3625204 Unknow CNBD1 - SYF2 ENSG00000264970
0.0054350 7.3625204 Unknow CNBD1 - SYF2 ENSG00000286353
0.0054350 7.3625204 Unknow CNBD1 - SYF2 FAM3B
0.0054350 7.3625204 Unknow CNBD1 - SYF2 GUSBP16
0.0054350 7.3625204 Unknow CNBD1 - SYF2 HAGH
0.0054350 7.3625204 Unknow CNBD1 - SYF2 LRRC8D
0.0054350 7.3625204 Unknow CNBD1 - SYF2 SLC2A1
0.0054350 7.3625204 Unknow CNBD1 - SYF2 SULT1C2P1
0.0054350 7.3625204 Unknow CNBD1 - SYF2 TMPRSS5
0.0000000 3.0865881 CGE PROX1 CNBD1 - PPFIBP2 PPFIBP2
0.0001626 6.8893839 CGE PROX1 CNBD1 - PPFIBP2 FAM3A
0.0001626 6.8750668 CGE PROX1 CNBD1 - PPFIBP2 SLC12A7
0.0005880 6.0175493 CGE PROX1 CNBD1 - PPFIBP2 PIGF
0.0005880 5.0419206 CGE PROX1 CNBD1 - PPFIBP2 VSTM1
0.0010938 0.7884376 CGE PROX1 CNBD1 - PPFIBP2 LINC01681
0.0026767 3.2572020 CGE PROX1 CNBD1 - PPFIBP2 TP73-AS1
0.0094712 3.9938405 CGE PROX1 CNBD1 - PPFIBP2 CCDC63
0.0095419 3.7123521 CGE PROX1 CNBD1 - PPFIBP2 ENSG00000230552
0.0000000 3.2843862 MGE SOX6 CNBD1 - KCNG4 KCNG4
0.0000960 0.7161892 MGE SOX6 CNBD1 - KCNG4 LINC01681
0.0007907 3.0922636 MGE SOX6 CNBD1 - KCNG4 CXXC4-AS1
0.0015167 2.0585607 MGE SOX6 CNBD1 - KCNG4 LINC01592
0.0015336 3.9286637 MGE SOX6 CNBD1 - KCNG4 ENSG00000259097
0.0015336 5.7671523 MGE SOX6 CNBD1 - KCNG4 XRRA1
0.0015336 4.4398513 MGE SOX6 CNBD1 - KCNG4 ENSG00000262898
0.0053327 1.1747842 MGE SOX6 CNBD1 - KCNG4 PABPC1
0.0053850 1.8389614 MGE SOX6 CNBD1 - KCNG4 RARB
0.0064817 2.1530220 MGE SOX6 CNBD1 - KCNG4 KNG1
0.0000000 3.1776279 Unknow CNBD1 - SUCO SUCO
0.0006812 4.2828772 Unknow CNBD1 - SUCO CENPV
0.0096259 1.4428880 Unknow CNBD1 - SUCO ZNF189
0.0054350 7.7816434 Unknow CNBD1 - IGF2R GPAT2
0.0054350 7.2163748 Unknow CNBD1 - IGF2R C1orf115
0.0054350 7.2163748 Unknow CNBD1 - IGF2R ENSG00000224626
0.0054350 7.2163748 Unknow CNBD1 - IGF2R ENSG00000286101
0.0054350 7.2163748 Unknow CNBD1 - IGF2R ENSG00000287189
0.0054350 7.2163748 Unknow CNBD1 - IGF2R RAD51AP2
0.0054350 7.1829518 Unknow CNBD1 - IGF2R ENSG00000228274
0.0054350 7.1829518 Unknow CNBD1 - IGF2R ENSG00000230896
0.0054350 7.1829518 Unknow CNBD1 - IGF2R ENSG00000287219
0.0054350 7.1829518 Unknow CNBD1 - IGF2R UBE3AP2
0.0054350 7.1829518 Unknow CNBD1 - IGF2R ZNF304
0.0054350 8.1203938 Unknow CNBD1 - RPS15P4 HAPLN1
0.0054350 7.4275440 Unknow CNBD1 - RPS15P4 AQP11
0.0054350 7.4275440 Unknow CNBD1 - RPS15P4 CDC42BPG
0.0054350 7.4275440 Unknow CNBD1 - RPS15P4 DRC3
0.0054350 7.4275440 Unknow CNBD1 - RPS15P4 ENSG00000231024
0.0054350 7.4275440 Unknow CNBD1 - RPS15P4 ENSG00000286823
0.0054350 7.4275440 Unknow CNBD1 - RPS15P4 LINC01878
0.0054350 7.4275440 Unknow CNBD1 - RPS15P4 MBD3L2B
0.0054350 7.4275440 Unknow CNBD1 - RPS15P4 S100A16
0.0054350 7.4275440 Unknow CNBD1 - RPS15P4 SRMS
0.0063155 6.5037890 MGE ETV1 CNBD1 - CSDE1 EEF1A1P28
0.0063155 6.5037890 MGE ETV1 CNBD1 - CSDE1 ENSG00000257767
0.0063155 6.5037890 MGE ETV1 CNBD1 - CSDE1 LHFPL1
0.0063155 6.5037890 MGE ETV1 CNBD1 - CSDE1 LOH12CR2
0.0063155 6.5037890 MGE ETV1 CNBD1 - CSDE1 MAPK15
0.0063155 6.5037890 MGE ETV1 CNBD1 - CSDE1 MIR210HG
0.0063155 6.5037890 MGE ETV1 CNBD1 - CSDE1 PLAGL2
0.0063155 6.5037890 MGE ETV1 CNBD1 - CSDE1 VWDE
0.0063155 6.5037890 MGE ETV1 CNBD1 - CSDE1 ZBTB22
0.0063155 6.9684468 MGE ETV1 CNBD1 - CSDE1 LINC00032
0.0063155 6.8948659 MGE ETV1 CNBD1 - CSDE1 ENSG00000225792
0.0063155 6.8948659 MGE ETV1 CNBD1 - CSDE1 ENSG00000259001
0.0063155 6.8948659 MGE ETV1 CNBD1 - CSDE1 PCNPP1
0.0063155 6.8948659 MGE ETV1 CNBD1 - CSDE1 PRPF39-DT
0.0045362 7.3239305 Unknow CNBD1 - RPS15AP1 ADAMTS7
0.0045362 6.9087548 Unknow CNBD1 - RPS15AP1 BARX1
0.0045362 6.9087548 Unknow CNBD1 - RPS15AP1 CDK2AP2
0.0045362 6.9087548 Unknow CNBD1 - RPS15AP1 HLA-H
0.0045362 6.9087548 Unknow CNBD1 - RPS15AP1 POU5F1B
0.0045362 6.9087548 Unknow CNBD1 - RPS15AP1 RPS16P2
0.0045362 6.9087548 Unknow CNBD1 - RPS15AP1 SNRPEP4
0.0045362 6.9087548 Unknow CNBD1 - RPS15AP1 TMED6
0.0045362 7.0594378 Unknow CNBD1 - RPS15AP1 ASB2
0.0045362 7.0594378 Unknow CNBD1 - RPS15AP1 IQCA1L
0.0045362 7.0594378 Unknow CNBD1 - RPS15AP1 LINC01443
0.0045362 7.4599787 Unknow CNBD1 - RPS15AP1 ENSG00000265380
0.0045362 7.4599787 Unknow CNBD1 - RPS15AP1 GFI1
0.0045362 7.4599787 Unknow CNBD1 - RPS15AP1 LINC02829
0.0045362 7.4599787 Unknow CNBD1 - RPS15AP1 LTK
0.0045362 7.4599787 Unknow CNBD1 - RPS15AP1 MT-TH
0.0071823 7.4275440 Unknow CNBD1 - ENSG00000185641 LAPTM4A-DT
0.0071823 7.4275440 Unknow CNBD1 - ENSG00000185641 PCDHAC1
0.0071823 7.0184657 Unknow CNBD1 - ENSG00000185641 WFDC8
0.0071823 6.9433123 Unknow CNBD1 - ENSG00000185641 ANKRD30A
0.0071823 6.9433123 Unknow CNBD1 - ENSG00000185641 ENSG00000289258
0.0071823 6.9433123 Unknow CNBD1 - ENSG00000185641 LINC01771
0.0071823 6.9433123 Unknow CNBD1 - ENSG00000185641 LINC02005
0.0071823 6.7349913 Unknow CNBD1 - ENSG00000185641 ENPP5
0.0071823 6.7349913 Unknow CNBD1 - ENSG00000185641 ENSG00000264895
0.0071823 6.7349913 Unknow CNBD1 - ENSG00000185641 ENSG00000269053
0.0071823 6.7349913 Unknow CNBD1 - ENSG00000185641 ENSG00000283234
0.0071823 6.7349913 Unknow CNBD1 - ENSG00000185641 IDI1P1
0.0097258 7.1829518 Unknow CNBD1 - DCLK1 SLC46A2
0.0097258 6.6466902 Unknow CNBD1 - DCLK1 ENSG00000260293
0.0097258 6.6466902 Unknow CNBD1 - DCLK1 ENSG00000289292
0.0097258 7.6775270 Unknow CNBD1 - DCLK1 LINC02236
0.0097258 7.0140154 Unknow CNBD1 - DCLK1 CFAP298-TCP10L
0.0097258 7.0140154 Unknow CNBD1 - DCLK1 ENSG00000240521
0.0097258 7.0140154 Unknow CNBD1 - DCLK1 HKDC1
0.0097258 7.0140154 Unknow CNBD1 - DCLK1 LINC00189
0.0097258 7.0140154 Unknow CNBD1 - DCLK1 OR5V1
0.0097258 6.5798406 Unknow CNBD1 - DCLK1 DENND3-AS1
0.0097258 6.5798406 Unknow CNBD1 - DCLK1 ENSG00000250068
0.0097258 6.5798406 Unknow CNBD1 - DCLK1 ENSG00000287984
0.0097258 6.5798406 Unknow CNBD1 - DCLK1 PNLIP
0.0097258 6.5798406 Unknow CNBD1 - DCLK1 RPL5P3
0.0097258 6.5798406 Unknow CNBD1 - DCLK1 WNK4
0.0063155 6.5613343 CGE PROX1 FNBP4 - IP6K2 ATP8B3
0.0063155 6.5613343 CGE PROX1 FNBP4 - IP6K2 RPS17P5
0.0063155 6.5613343 CGE PROX1 FNBP4 - IP6K2 SPDEF
0.0063155 6.5613343 CGE PROX1 FNBP4 - IP6K2 TMEM209
0.0063155 6.9812555 CGE PROX1 FNBP4 - IP6K2 RRAS
0.0063155 6.6684360 CGE PROX1 FNBP4 - IP6K2 ENSG00000234244
0.0063155 6.6684360 CGE PROX1 FNBP4 - IP6K2 MTCYBP16
0.0063155 6.6684360 CGE PROX1 FNBP4 - IP6K2 PHF2P2
0.0063155 6.6684360 CGE PROX1 FNBP4 - IP6K2 RGPD4-AS1
0.0063155 6.6684360 CGE PROX1 FNBP4 - IP6K2 SORD
0.0063155 6.7316396 CGE PROX1 FNBP4 - IP6K2 RPS4XP6
0.0080382 7.2940975 CTX NEUROD2 CNBD1 - ZC3H13 CENPB
0.0080382 6.9248682 CTX NEUROD2 CNBD1 - ZC3H13 ENSG00000264622
0.0080382 6.9248682 CTX NEUROD2 CNBD1 - ZC3H13 FAM90A10P
0.0080382 6.9248682 CTX NEUROD2 CNBD1 - ZC3H13 ITGAD
0.0080382 6.9248682 CTX NEUROD2 CNBD1 - ZC3H13 ZSCAN32
0.0080382 6.8772881 CTX NEUROD2 CNBD1 - ZC3H13 DEFB128
0.0080382 6.7400400 CTX NEUROD2 CNBD1 - ZC3H13 ANGPTL4
0.0080382 6.7400400 CTX NEUROD2 CNBD1 - ZC3H13 LINC02273
0.0080382 6.7400400 CTX NEUROD2 CNBD1 - ZC3H13 LINC02656
0.0080382 6.7400400 CTX NEUROD2 CNBD1 - ZC3H13 OPLAH
0.0080382 6.7400400 CTX NEUROD2 CNBD1 - ZC3H13 TRBV22-1
0.0045362 7.8446406 Unknow CNBD1 - DLG2 TOPAZ1
0.0045362 7.1518852 Unknow CNBD1 - DLG2 AKR7L
0.0045362 7.1518852 Unknow CNBD1 - DLG2 ASB5
0.0045362 7.1518852 Unknow CNBD1 - DLG2 CCKBR
0.0045362 7.1518852 Unknow CNBD1 - DLG2 ENSG00000229528
0.0045362 7.1518852 Unknow CNBD1 - DLG2 ENSG00000231953
0.0045362 7.1518852 Unknow CNBD1 - DLG2 ENSG00000287853
0.0045362 7.1518852 Unknow CNBD1 - DLG2 PGGHG
0.0045362 7.1518852 Unknow CNBD1 - DLG2 PTPRB
0.0045362 7.4059435 Unknow CNBD1 - DLG2 ENSG00000285791
0.0045362 7.4059435 Unknow CNBD1 - DLG2 FAM110D
0.0045362 7.4059435 Unknow CNBD1 - DLG2 LINC01088
0.0045362 7.4059435 Unknow CNBD1 - DLG2 ZNF174
0.0045362 7.6339095 Unknow CNBD1 - DLG2 DCLK3
0.0045362 7.6339095 Unknow CNBD1 - DLG2 LINC02091
0.0045362 7.6339095 Unknow CNBD1 - DLG2 PKDCC
0.0045362 7.6339095 Unknow CNBD1 - DLG2 VIPR1
0.0054350 7.6632478 Unknow CNBD1 - YWHAZ CSRNP1
0.0054350 7.6632478 Unknow CNBD1 - YWHAZ ENSG00000260861
0.0054350 7.6632478 Unknow CNBD1 - YWHAZ PTPN6
0.0054350 6.8552690 Unknow CNBD1 - YWHAZ EIF1AXP1
0.0054350 6.8552690 Unknow CNBD1 - YWHAZ ST13P12
0.0054350 6.8318737 Unknow CNBD1 - YWHAZ LINC02826
0.0054350 6.7778664 Unknow CNBD1 - YWHAZ ENSG00000282478
0.0054350 6.7778664 Unknow CNBD1 - YWHAZ KIAA0895LP1
0.0054350 6.7778664 Unknow CNBD1 - YWHAZ MFSD12
0.0054350 6.7778664 Unknow CNBD1 - YWHAZ SOX8
0.0054350 7.4530623 Unknow CNBD1 - YWHAZ LINC01971
0.0054350 7.4530623 Unknow CNBD1 - YWHAZ TDRD15



Heatmap of cell subtypes with specific markers
Log(CPM + 1) normalization of expression


Heterogeneity of cells within subtypes: number of genes vs. mean expression



Disclaimer

The analysis was performed through the non-profit and open-source pipeline JSEQ_scRNAseq©. Pipeline belongs to the part of the JBioSystem© project in which we create complex software for bioinformatics analysis. Remember in silico analysis can not be taken for granted and must be validated in vitro or in vivo. For more information go to GitHub or contact us. The JSEQ_scRNAseq© was created in the Department of Molecular Neurobiology, Institute of Bioorganic Chemistry, Polish Academy of Sciences